Journal: Science Advances

Article Title: Targeting macrophage necroptosis for therapeutic and diagnostic interventions in atherosclerosis

doi: 10.1126/sciadv.1600224

Figure Lengend Snippet: ( A ) mRNA expression of RIP3 and MLKL in carotid endarterectomies (plaque) or macroscopically disease-free control arteries (normal). ( B ) RIP3 and MLKL mRNA expression in plaque samples from (A) classified as asymptomatic (stable) or symptomatic patients (unstable) [from the Biobank of Karolinska Endarterectomies (BiKE)]. * P ≤ 0.05, ** P < 0.01, **** P < 0.0001 by Student’s t test. ( C ) Immunohistochemical analysis of pMLKL in human coronary arteries with early lesions with pathologic intimal thickening ( n = 5 arterial segments) and advanced fibroatheroma lesions ( n = 11 arterial segments). The graph depicts quantification of the pMLKL-positive area. * P ≤ 0.05. H&E, hematoxylin and eosin.

Article Snippet: For immunohistochemical analysis, human coronary artery samples were obtained from the CVPath Institute Sudden Cardiac Death registry, as previously described ( ).

Techniques: Expressing, Control, Immunohistochemical staining

Journal: Science Advances

Article Title: Targeting macrophage necroptosis for therapeutic and diagnostic interventions in atherosclerosis

doi: 10.1126/sciadv.1600224

Figure Lengend Snippet: ( A ) Apoe −/− mice were fed a Western diet for 6 weeks before implantation of time-release pellets containing placebo or Nec-1s (2 mg/kg per day). After four additional weeks of Western diet feeding, the mice were harvested for morphometric analysis of atherosclerosis. ( B ) En face lesion area was measured in placebo- and Nec-1s–treated mice and is represented as lesion area as a percentage of total aorta area. ( C ) Lesion area in the aortic sinus in placebo- and Nec-1s–treated mice is represented as total area in μm 2 . ( D ) Necrotic core area within aortic sinus lesions. ( E to G ) Immunohistochemical staining of smooth muscle-α actin (SMC marker) (E), CD68 (macrophage marker) (F), and pMLKL (G) was performed on aortic sinus lesions and quantified with ImageJ. Representative images per group are shown. AU, arbitrary units. ( H ) Serum IL-1β in mice from placebo- or Nec-1–treated groups was measured at sacrifice by enzyme-linked immunosorbent assay (ELISA). * P ≤ 0.05, ** P < 0.01 by Student’s t test.

Article Snippet: For immunohistochemical analysis, human coronary artery samples were obtained from the CVPath Institute Sudden Cardiac Death registry, as previously described ( ).

Techniques: Western Blot, Immunohistochemical staining, Staining, Marker, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Pharmacology

Article Title: Protective effects of Ganoderma lucidum spores on estradiol benzoate-induced TEC apoptosis and compromised double-positive thymocyte development

doi: 10.3389/fphar.2024.1419881

Figure Lengend Snippet: The co-localization of cytokeratin (CK) and Casp3 were analyzed using immunohistochemistry (IHC) staining in thymus tissues. (A,B) The co-localization of CK8 and Casp3 (A) and relative quantitative analysis (B) . (C,D) The co-localization of CK5 and Casp3 (C) and relative quantitative analysis (D) . Scale bar = 500 μm (40 ×), 50 μm (400 ×), n = 6.

Article Snippet: In briefly, formalin-fixed thymus tissues were embedded in paraffin and cut into 4-μm sections consecutively, and 2 adjacent sections were selected for co-localized IHC analysis of cytokeratin5 (CK5, Proteintech, Cat: 19677-1-AP)-Caspase 3 (Casp3, Proteintech, Cat: 19677-1-AP), cytokeratin8 (CK8, Proteintech, Cat: 17514-1-AP)-Casp3, and CK5-Aire (Proteintech, Cat: 22517-1-AP).

Techniques: Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Protective effects of Ganoderma lucidum spores on estradiol benzoate-induced TEC apoptosis and compromised double-positive thymocyte development

doi: 10.3389/fphar.2024.1419881

Figure Lengend Snippet: The co-localization of CK5 and Aire were analyzed using IHC staining in thymus tissues, and relative quantitative analysis. Scale bar = 500 μm (40 ×), 50 μm (400 ×), n = 6.

Article Snippet: In briefly, formalin-fixed thymus tissues were embedded in paraffin and cut into 4-μm sections consecutively, and 2 adjacent sections were selected for co-localized IHC analysis of cytokeratin5 (CK5, Proteintech, Cat: 19677-1-AP)-Caspase 3 (Casp3, Proteintech, Cat: 19677-1-AP), cytokeratin8 (CK8, Proteintech, Cat: 17514-1-AP)-Casp3, and CK5-Aire (Proteintech, Cat: 22517-1-AP).

Techniques: Immunohistochemistry

Journal: American Journal of Cancer Research

Article Title: hsa_circ_0001741 promotes esophageal squamous cell carcinoma stemness, invasion and migration by sponging miR-491-5p to upregulate NOTCH3 expression

doi:

Figure Lengend Snippet: Ablation of hsa_circ_0001741 inhibits tumorigenicity of ESCC cells. (A) In vivo limiting dilution analysis of TE1 cells with or without hsa_circ_0001741 knockdown (n = 6). (B) The frequency of tumor formation at each cell dose injected was shown. The data were analyzed using ELDA software. (C, D) Tumor volumes (C) were measured with a caliper over a 7-week time period, and tumor weights (D) were also measured at the end of the study, with both graphs generated using data from mice receiving 1 × 106 injected tumor cells. (E) Expression levels of NOTCH3, SOX2, Nanog, N-cadherin E-cadherin and in tumors were analyzed by IHC staining. **P < 0.01, ***P < 0.001.

Article Snippet: After the mice were sacrificed, tumors were subjected to further immunohistochemical analysis using primary antibodies against SOX2 (Proteintech, China), Nanog (Proteintech, China), N-cadherin (Immunoway, China), E-cadherin (Immunoway, China) and NOTCH3 (Proteintech, China).

Techniques: In Vivo, Knockdown, Injection, Software, Generated, Expressing, Immunohistochemistry